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Cell Signaling Technology Inc aib1 antibody

Mouse AIB1Δ5 is a homolog of human AIB1Δ4 (A) Representative Sashimi plot of RNA sequencing reads skipping exon 5 (blue arrow) in mouse E0771 cells. (B) Expression level of the GR-regulated gene Fkbp5 in E0771 empty vector (E0771-EV) and E0771-AIB1Δ5 overexpressing (E0771-Δ5) cells treated with either 10 nM dex or 1 μM rela for 48 h ( n = 3 technical replicates per condition). Data are represented as mean ± SD. ∗ p < 0.05. ∗∗∗ p < 0.001. p value was calculated using two-tailed unpaired Student’s t-tests. (C) Relative level of AIB1Δ5 mRNA in indicated E0771 samples, normalized to full-length <t>AIB1.</t> Normal lung samples are from control female C57BL/6 mice without E0771 tumor cell injection. Data represent n = 4 biological replicates for E0771 cells grown in culture, n = 3 biological replicates for tumor samples, n = 3 biological replicates for lung metastasis, and n = 3 biological replicates for tumor naive lung tissue. Data are represented as mean ± SD. ∗ p < 0.05. p value was calculated using two-tailed unpaired Student’s t test. (D) Expression level of AIB1 and AIB1Δ5 mRNA using RT-qPCR in the indicated samples. Tumor and immune cells were dissociated from primary tumor and spleen tissues and FACS sorted for CD45 − , CD45 + , or CD45+/CD3+ cells prior to RNA extraction and RT-qPCR. Median plotted as a solid line. Data represent n = 4 biological replicates for E0771 cells grown in culture, n = 8 tumor samples ( n = 4 mice), and n = 3 mouse spleens. ∗∗ p < 0.01. ∗∗∗ p < 0.001. ∗∗∗∗ p < 0.0001. p value was calculated using two-tailed unpaired Student’s t test.

Aib1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progression"

Article Title: An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progression

Journal: iScience

doi: 10.1016/j.isci.2026.114768

Figure Legend Snippet: Mouse AIB1Δ5 is a homolog of human AIB1Δ4 (A) Representative Sashimi plot of RNA sequencing reads skipping exon 5 (blue arrow) in mouse E0771 cells. (B) Expression level of the GR-regulated gene Fkbp5 in E0771 empty vector (E0771-EV) and E0771-AIB1Δ5 overexpressing (E0771-Δ5) cells treated with either 10 nM dex or 1 μM rela for 48 h ( n = 3 technical replicates per condition). Data are represented as mean ± SD. ∗ p < 0.05. ∗∗∗ p < 0.001. p value was calculated using two-tailed unpaired Student’s t-tests. (C) Relative level of AIB1Δ5 mRNA in indicated E0771 samples, normalized to full-length AIB1. Normal lung samples are from control female C57BL/6 mice without E0771 tumor cell injection. Data represent n = 4 biological replicates for E0771 cells grown in culture, n = 3 biological replicates for tumor samples, n = 3 biological replicates for lung metastasis, and n = 3 biological replicates for tumor naive lung tissue. Data are represented as mean ± SD. ∗ p < 0.05. p value was calculated using two-tailed unpaired Student’s t test. (D) Expression level of AIB1 and AIB1Δ5 mRNA using RT-qPCR in the indicated samples. Tumor and immune cells were dissociated from primary tumor and spleen tissues and FACS sorted for CD45 − , CD45 + , or CD45+/CD3+ cells prior to RNA extraction and RT-qPCR. Median plotted as a solid line. Data represent n = 4 biological replicates for E0771 cells grown in culture, n = 8 tumor samples ( n = 4 mice), and n = 3 mouse spleens. ∗∗ p < 0.01. ∗∗∗ p < 0.001. ∗∗∗∗ p < 0.0001. p value was calculated using two-tailed unpaired Student’s t test.

Techniques Used: RNA Sequencing, Expressing, Plasmid Preparation, Two Tailed Test, Control, Injection, Quantitative RT-PCR, RNA Extraction

Glucocorticoid transcriptional responses are altered by the switch to AIBΔ4 expression in TNBC cells (A) Luciferase assay using MMTV promoter-driven firefly luciferase. HEK 293T cells were transfected with an MMTV promoter-driven firefly construct, a human GR expression vector, and either a pcDNA3 empty vector, pcDNA3-AIB1, or pcDNA3-AIB1Δ4 expression vector. Cells were treated with either vehicle control or 10 nM dex. Luciferase activity was measured 24 h after transfection. Dot blot shows AIB1 protein levels in the three samples treated with dex. Below the dot blot are the protein expression levels normalized to pcDNA3-empty vector. Data are represented as mean ± SD ( n = 3). RLU = relative light unit. ∗∗∗∗ p < 0.0001. p value was calculated using two-tailed unpaired Student’s t-tests. (B) Western blot for GR in DCIS and DCISΔ4 cells after overnight treatment with vehicle, 5 nM dex, or 5 nM dex plus 500 nM RU486. (C) Differentially expressed genes (RNA-seq) of dex (5 nM) treated versus dex (5 nM) plus RU486 (500 nM) treated DCIS or DCISΔ4 cells. All differentially expressed genes had an FDR ≤0.05. Data represent n = 3 biological replicates for each cell line and treatment condition. (D) Bar graph depicting differentially expressed genes in B that are either commonly or uniquely activated or repressed in the DCIS and DCISΔ4 cells. (E) Western blot for GR in DCIS and DCISΔ4 cells after 1 h treatment with vehicle, 5 nM dex or 5 nM dex plus 500 nM RU486. Cells were cultured in media without serum and hydrocortisone for 4 h prior to drug treatment in full serum. (F) Peak overlap from a CUT&RUN experiment with a GR antibody in DCIS and DCISΔ4 cells. Cells were treated for 1 h with dex prior to conducting the CUT&RUN experiment. Data represent n = 3 biological replicates for each cell line. Peaks were called with SEACR in stringent mode and MACS3 with a q-value cutoff of ≤0.05. Consensus peaks were those called in at least 2 of the 3 replicates. (G and H) Categorization of significantly enriched motifs from the GR CUT&RUN experiment in DCIS (E) and DCISΔ4 (F) cells. bZIP = basic leucine zipper; bHLH = basic-helix-loop-helix; HMG = high-mobility group; Zf = zinc finger; NR = nuclear receptor. (I) Heatmap representation of ATAC-seq peaks found in DCIS and DCISΔ4 cells. Cells were treated for 1 h with dex prior to the ATAC-seq experiment. Overlap peaks were called in both cell lines ( n = 87,356). DCIS enriched peaks were only called in the DCIS parental cell line ( n = 5,459), and DCISΔ4 enriched peaks were only called in the DCISΔ4 cell line ( n = 60,715). Data represent n = 3 biological replicates for each cell line. Peaks were called with MACS3 with a q-value cutoff of ≤0.01. Consensus peaks were those called in at least 2 of the 3 replicates. (J) GR CUT&RUN reads (RPKM) in DCIS and DCISΔ4 cells plotted at ATAC-seq peak loci from G.

Figure Legend Snippet: Glucocorticoid transcriptional responses are altered by the switch to AIBΔ4 expression in TNBC cells (A) Luciferase assay using MMTV promoter-driven firefly luciferase. HEK 293T cells were transfected with an MMTV promoter-driven firefly construct, a human GR expression vector, and either a pcDNA3 empty vector, pcDNA3-AIB1, or pcDNA3-AIB1Δ4 expression vector. Cells were treated with either vehicle control or 10 nM dex. Luciferase activity was measured 24 h after transfection. Dot blot shows AIB1 protein levels in the three samples treated with dex. Below the dot blot are the protein expression levels normalized to pcDNA3-empty vector. Data are represented as mean ± SD ( n = 3). RLU = relative light unit. ∗∗∗∗ p < 0.0001. p value was calculated using two-tailed unpaired Student’s t-tests. (B) Western blot for GR in DCIS and DCISΔ4 cells after overnight treatment with vehicle, 5 nM dex, or 5 nM dex plus 500 nM RU486. (C) Differentially expressed genes (RNA-seq) of dex (5 nM) treated versus dex (5 nM) plus RU486 (500 nM) treated DCIS or DCISΔ4 cells. All differentially expressed genes had an FDR ≤0.05. Data represent n = 3 biological replicates for each cell line and treatment condition. (D) Bar graph depicting differentially expressed genes in B that are either commonly or uniquely activated or repressed in the DCIS and DCISΔ4 cells. (E) Western blot for GR in DCIS and DCISΔ4 cells after 1 h treatment with vehicle, 5 nM dex or 5 nM dex plus 500 nM RU486. Cells were cultured in media without serum and hydrocortisone for 4 h prior to drug treatment in full serum. (F) Peak overlap from a CUT&RUN experiment with a GR antibody in DCIS and DCISΔ4 cells. Cells were treated for 1 h with dex prior to conducting the CUT&RUN experiment. Data represent n = 3 biological replicates for each cell line. Peaks were called with SEACR in stringent mode and MACS3 with a q-value cutoff of ≤0.05. Consensus peaks were those called in at least 2 of the 3 replicates. (G and H) Categorization of significantly enriched motifs from the GR CUT&RUN experiment in DCIS (E) and DCISΔ4 (F) cells. bZIP = basic leucine zipper; bHLH = basic-helix-loop-helix; HMG = high-mobility group; Zf = zinc finger; NR = nuclear receptor. (I) Heatmap representation of ATAC-seq peaks found in DCIS and DCISΔ4 cells. Cells were treated for 1 h with dex prior to the ATAC-seq experiment. Overlap peaks were called in both cell lines ( n = 87,356). DCIS enriched peaks were only called in the DCIS parental cell line ( n = 5,459), and DCISΔ4 enriched peaks were only called in the DCISΔ4 cell line ( n = 60,715). Data represent n = 3 biological replicates for each cell line. Peaks were called with MACS3 with a q-value cutoff of ≤0.01. Consensus peaks were those called in at least 2 of the 3 replicates. (J) GR CUT&RUN reads (RPKM) in DCIS and DCISΔ4 cells plotted at ATAC-seq peak loci from G.

Techniques Used: Expressing, Luciferase, Transfection, Construct, Plasmid Preparation, Control, Activity Assay, Dot Blot, Two Tailed Test, Western Blot, RNA Sequencing, Cell Culture

AIB1Δ4 chromatin engagement is affected by cell-cell crosstalk (A) Heatmap representation of peaks called from a CUT&RUN experiment with an AIB1 antibody in DCIS and DCISΔ4 cells. Cells were treated for 1 h with dex prior to conducting the CUT&RUN experiment. Data represent n = 3 biological replicates for each cell line. Peaks were called with SEACR in relaxed mode and MACS3 with a p value cutoff of ≤0.005. Consensus peaks were those called in at least 2 of the 3 replicates. (B) Top ten significantly enriched motifs found in the DCISΔ4 cell line from AIB1 CUT&RUN. (C and D) Peak overlap from a CUT&RUN experiment with an AIB1 antibody in DCISΔ4 and DCISΔ4 unmixed cells (C) and DCIS and DCIS unmixed cells (D). Cells were treated for 1 h with dex prior to conducting the CUT&RUN experiment. Data represent n = 3 biological replicates for each cell line. Peaks were called with SEACR in relaxed mode and MACS3 with a p value cutoff of ≤0.005. Consensus peaks were those called in at least 2 of the 3 replicates. (E) Top six significantly enriched motifs found in the DCISΔ4 unmixed cell line from AIB1 CUT&RUN. (F) Top human Enrichr ChEA 2022 terms for the 467 DCISΔ4 unmixed upregulated genes with dex treatment that also have AIB1 binding in DCISΔ4 unmixed cells (from H). The ChEA dataset is from target genes of transcription factors from published ChIP-chip, ChIP-seq, and other transcription factor binding site profiling studies.

Figure Legend Snippet: AIB1Δ4 chromatin engagement is affected by cell-cell crosstalk (A) Heatmap representation of peaks called from a CUT&RUN experiment with an AIB1 antibody in DCIS and DCISΔ4 cells. Cells were treated for 1 h with dex prior to conducting the CUT&RUN experiment. Data represent n = 3 biological replicates for each cell line. Peaks were called with SEACR in relaxed mode and MACS3 with a p value cutoff of ≤0.005. Consensus peaks were those called in at least 2 of the 3 replicates. (B) Top ten significantly enriched motifs found in the DCISΔ4 cell line from AIB1 CUT&RUN. (C and D) Peak overlap from a CUT&RUN experiment with an AIB1 antibody in DCISΔ4 and DCISΔ4 unmixed cells (C) and DCIS and DCIS unmixed cells (D). Cells were treated for 1 h with dex prior to conducting the CUT&RUN experiment. Data represent n = 3 biological replicates for each cell line. Peaks were called with SEACR in relaxed mode and MACS3 with a p value cutoff of ≤0.005. Consensus peaks were those called in at least 2 of the 3 replicates. (E) Top six significantly enriched motifs found in the DCISΔ4 unmixed cell line from AIB1 CUT&RUN. (F) Top human Enrichr ChEA 2022 terms for the 467 DCISΔ4 unmixed upregulated genes with dex treatment that also have AIB1 binding in DCISΔ4 unmixed cells (from H). The ChEA dataset is from target genes of transcription factors from published ChIP-chip, ChIP-seq, and other transcription factor binding site profiling studies.

Techniques Used: Binding Assay, ChIP-chip, ChIP-sequencing

Lysis:

Article Title: An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progression
Article Snippet: .. 24 hours later, whole 940 cell lysate was prepared with NP40 lysis buffer and 1 mg of protein was used for 941 immunoprecipitation with AIB1 antibody (Cell Signaling Technology, 2126). .. Pierce Protein A/G 942 Jo urn al Pr e-p roo f 22 Plus Agarose Beads (Thermo Scientific, 20423) were washed 3X with lysis buffer.

Immunoprecipitation:

other:

Article Title: An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progression
Article Snippet: Protein was detected with Immobilon Western Chemiluminescent HRP Substrate (Millipore, WBKLS0500).

Article Title: Regulation of HER2 Oncogene Transcription by a Multifunctional Coactivator/Corepressor Complex
Article Snippet: Immunoprecipitation and immunoblotting Immunoblotting and immunoprecipitation were performed with the following antibodies: Flag M2 (Sigma), GFP (Zymed), AIB1 (Cell Signaling), ANCO1 (Abcam), phospho-AKT (Cell Signaling), AKT (Cell Signaling), phospho-ERK (Cell Signaling), and ERK (Cell Signaling).

Article Title: Role of the Nuclear Receptor Coactivator AIB1/SRC-3 in Angiogenesis and Wound Healing
Article Snippet: Immunohistochemical Analyses Immunohistochemical analyses for proliferating cell nuclear antigen (PNCA; Sigma-Aldrich), F4/80 (AbD Serotec, Raleigh, NC), vascular endothelial growth factor-A (VEGF-A; Santa Cruz Biotechnology, Santa Cruz, CA), and AIB1 (Cell Signaling Technology, Danvers, MA) were performed on wound sections as previously described.

Image Search Results

Journal: iScience

Article Title: An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progression

doi: 10.1016/j.isci.2026.114768

Figure Lengend Snippet: Mouse AIB1Δ5 is a homolog of human AIB1Δ4 (A) Representative Sashimi plot of RNA sequencing reads skipping exon 5 (blue arrow) in mouse E0771 cells. (B) Expression level of the GR-regulated gene Fkbp5 in E0771 empty vector (E0771-EV) and E0771-AIB1Δ5 overexpressing (E0771-Δ5) cells treated with either 10 nM dex or 1 μM rela for 48 h ( n = 3 technical replicates per condition). Data are represented as mean ± SD. ∗ p < 0.05. ∗∗∗ p < 0.001. p value was calculated using two-tailed unpaired Student’s t-tests. (C) Relative level of AIB1Δ5 mRNA in indicated E0771 samples, normalized to full-length AIB1. Normal lung samples are from control female C57BL/6 mice without E0771 tumor cell injection. Data represent n = 4 biological replicates for E0771 cells grown in culture, n = 3 biological replicates for tumor samples, n = 3 biological replicates for lung metastasis, and n = 3 biological replicates for tumor naive lung tissue. Data are represented as mean ± SD. ∗ p < 0.05. p value was calculated using two-tailed unpaired Student’s t test. (D) Expression level of AIB1 and AIB1Δ5 mRNA using RT-qPCR in the indicated samples. Tumor and immune cells were dissociated from primary tumor and spleen tissues and FACS sorted for CD45 − , CD45 + , or CD45+/CD3+ cells prior to RNA extraction and RT-qPCR. Median plotted as a solid line. Data represent n = 4 biological replicates for E0771 cells grown in culture, n = 8 tumor samples ( n = 4 mice), and n = 3 mouse spleens. ∗∗ p < 0.01. ∗∗∗ p < 0.001. ∗∗∗∗ p < 0.0001. p value was calculated using two-tailed unpaired Student’s t test.

Article Snippet: 24 hours later, whole cell lysate was prepared with NP40 lysis buffer and 1 mg of protein was used for immunoprecipitation with AIB1 antibody (Cell Signaling Technology, 2126).

Techniques: RNA Sequencing, Expressing, Plasmid Preparation, Two Tailed Test, Control, Injection, Quantitative RT-PCR, RNA Extraction

Journal: iScience

Article Title: An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progression

doi: 10.1016/j.isci.2026.114768

Figure Lengend Snippet: Glucocorticoid transcriptional responses are altered by the switch to AIBΔ4 expression in TNBC cells (A) Luciferase assay using MMTV promoter-driven firefly luciferase. HEK 293T cells were transfected with an MMTV promoter-driven firefly construct, a human GR expression vector, and either a pcDNA3 empty vector, pcDNA3-AIB1, or pcDNA3-AIB1Δ4 expression vector. Cells were treated with either vehicle control or 10 nM dex. Luciferase activity was measured 24 h after transfection. Dot blot shows AIB1 protein levels in the three samples treated with dex. Below the dot blot are the protein expression levels normalized to pcDNA3-empty vector. Data are represented as mean ± SD ( n = 3). RLU = relative light unit. ∗∗∗∗ p < 0.0001. p value was calculated using two-tailed unpaired Student’s t-tests. (B) Western blot for GR in DCIS and DCISΔ4 cells after overnight treatment with vehicle, 5 nM dex, or 5 nM dex plus 500 nM RU486. (C) Differentially expressed genes (RNA-seq) of dex (5 nM) treated versus dex (5 nM) plus RU486 (500 nM) treated DCIS or DCISΔ4 cells. All differentially expressed genes had an FDR ≤0.05. Data represent n = 3 biological replicates for each cell line and treatment condition. (D) Bar graph depicting differentially expressed genes in B that are either commonly or uniquely activated or repressed in the DCIS and DCISΔ4 cells. (E) Western blot for GR in DCIS and DCISΔ4 cells after 1 h treatment with vehicle, 5 nM dex or 5 nM dex plus 500 nM RU486. Cells were cultured in media without serum and hydrocortisone for 4 h prior to drug treatment in full serum. (F) Peak overlap from a CUT&RUN experiment with a GR antibody in DCIS and DCISΔ4 cells. Cells were treated for 1 h with dex prior to conducting the CUT&RUN experiment. Data represent n = 3 biological replicates for each cell line. Peaks were called with SEACR in stringent mode and MACS3 with a q-value cutoff of ≤0.05. Consensus peaks were those called in at least 2 of the 3 replicates. (G and H) Categorization of significantly enriched motifs from the GR CUT&RUN experiment in DCIS (E) and DCISΔ4 (F) cells. bZIP = basic leucine zipper; bHLH = basic-helix-loop-helix; HMG = high-mobility group; Zf = zinc finger; NR = nuclear receptor. (I) Heatmap representation of ATAC-seq peaks found in DCIS and DCISΔ4 cells. Cells were treated for 1 h with dex prior to the ATAC-seq experiment. Overlap peaks were called in both cell lines ( n = 87,356). DCIS enriched peaks were only called in the DCIS parental cell line ( n = 5,459), and DCISΔ4 enriched peaks were only called in the DCISΔ4 cell line ( n = 60,715). Data represent n = 3 biological replicates for each cell line. Peaks were called with MACS3 with a q-value cutoff of ≤0.01. Consensus peaks were those called in at least 2 of the 3 replicates. (J) GR CUT&RUN reads (RPKM) in DCIS and DCISΔ4 cells plotted at ATAC-seq peak loci from G.

Article Snippet: 24 hours later, whole cell lysate was prepared with NP40 lysis buffer and 1 mg of protein was used for immunoprecipitation with AIB1 antibody (Cell Signaling Technology, 2126).

Techniques: Expressing, Luciferase, Transfection, Construct, Plasmid Preparation, Control, Activity Assay, Dot Blot, Two Tailed Test, Western Blot, RNA Sequencing, Cell Culture

Journal: iScience

Article Title: An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progression

doi: 10.1016/j.isci.2026.114768

Figure Lengend Snippet: AIB1Δ4 chromatin engagement is affected by cell-cell crosstalk (A) Heatmap representation of peaks called from a CUT&RUN experiment with an AIB1 antibody in DCIS and DCISΔ4 cells. Cells were treated for 1 h with dex prior to conducting the CUT&RUN experiment. Data represent n = 3 biological replicates for each cell line. Peaks were called with SEACR in relaxed mode and MACS3 with a p value cutoff of ≤0.005. Consensus peaks were those called in at least 2 of the 3 replicates. (B) Top ten significantly enriched motifs found in the DCISΔ4 cell line from AIB1 CUT&RUN. (C and D) Peak overlap from a CUT&RUN experiment with an AIB1 antibody in DCISΔ4 and DCISΔ4 unmixed cells (C) and DCIS and DCIS unmixed cells (D). Cells were treated for 1 h with dex prior to conducting the CUT&RUN experiment. Data represent n = 3 biological replicates for each cell line. Peaks were called with SEACR in relaxed mode and MACS3 with a p value cutoff of ≤0.005. Consensus peaks were those called in at least 2 of the 3 replicates. (E) Top six significantly enriched motifs found in the DCISΔ4 unmixed cell line from AIB1 CUT&RUN. (F) Top human Enrichr ChEA 2022 terms for the 467 DCISΔ4 unmixed upregulated genes with dex treatment that also have AIB1 binding in DCISΔ4 unmixed cells (from H). The ChEA dataset is from target genes of transcription factors from published ChIP-chip, ChIP-seq, and other transcription factor binding site profiling studies.

Article Snippet: 24 hours later, whole cell lysate was prepared with NP40 lysis buffer and 1 mg of protein was used for immunoprecipitation with AIB1 antibody (Cell Signaling Technology, 2126).

Techniques: Binding Assay, ChIP-chip, ChIP-sequencing

Figure 3. SIX1 promotes lipogenic gene expression through association with AIB1 and HBO1. A, B) qRT-PCR (A) and immunoblot (B) analysis of AIB1/HBO1 WT or KO HepG2 cells transfected with SIX1, HBO1, AIB1, or EV as indicated. C) ChIP analysis of SIX1, AIB1, and HBO1 occupancy on the promoters of lipogenic genes in HepG2 cells. Promoter regions of each gene represent the region containing the ﬁrst or second SIX1 binding site shown in Figure 2B within the gene promoters analyzed. D) Re-ChIP analysis of the occupancy of SIX1 and AIB1 or HBO1 on the indicated lipogenic gene promoters in HepG2 cells. E) ChIP analysis of SIX1, HBO1, AIB1, and histone H3 or H4 acetylation (ac) occupancy on the indicated promoters of lipogenic genes in SIX1, AIB1, or HBO1 KO HepG2 cells. Data shown are mean ± SD of triplicate measurements. Experiments have been repeated 3 times with similar results. A two-sided Student’s t-test was used to compare the means of 2 groups. When more than 2 groups were compared, one-way ANOVA was performed. **p < 0.01 versus respective WT HepG2 cells transfected with empty vector (A). *p < 0.05, **p < 0.01 versus respective normal IgG (C-E).

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells.

doi: 10.1002/advs.202404229

Figure Lengend Snippet: Figure 3. SIX1 promotes lipogenic gene expression through association with AIB1 and HBO1. A, B) qRT-PCR (A) and immunoblot (B) analysis of AIB1/HBO1 WT or KO HepG2 cells transfected with SIX1, HBO1, AIB1, or EV as indicated. C) ChIP analysis of SIX1, AIB1, and HBO1 occupancy on the promoters of lipogenic genes in HepG2 cells. Promoter regions of each gene represent the region containing the ﬁrst or second SIX1 binding site shown in Figure 2B within the gene promoters analyzed. D) Re-ChIP analysis of the occupancy of SIX1 and AIB1 or HBO1 on the indicated lipogenic gene promoters in HepG2 cells. E) ChIP analysis of SIX1, HBO1, AIB1, and histone H3 or H4 acetylation (ac) occupancy on the indicated promoters of lipogenic genes in SIX1, AIB1, or HBO1 KO HepG2 cells. Data shown are mean ± SD of triplicate measurements. Experiments have been repeated 3 times with similar results. A two-sided Student’s t-test was used to compare the means of 2 groups. When more than 2 groups were compared, one-way ANOVA was performed. **p < 0.01 versus respective WT HepG2 cells transfected with empty vector (A). *p < 0.05, **p < 0.01 versus respective normal IgG (C-E).

Article Snippet: The membranes were blocked with 5% skimmed milk for 1 h at room temperature, followed by incubation with primary antibodies including anti-ACLY (Proteintech; Cat# 67166-1-Ig), anti-ACC1 antibody (Proteintech; Cat# 21923-1-AP), anti-FASN antibody (Proteintech; Cat# 10624-2-AP), anti-SCD1 antibody (Proteintech; Cat# 28678-1-AP), anti-SIX1 antibody (Proteintech; Cat# 10709-1-AP), anti-AIB1 antibody (Santa Cruz Biotechnology; Cat# sc-9119), anti-HBO1 antibody (Proteintech; Cat# 13751-1-AP), and anti-β-actin (Santa Cruz Biotechnology; Cat# sc-47778HRP) at room temperature for 2 h or overnight at 4 °C.

Techniques: Gene Expression, Quantitative RT-PCR, Western Blot, Transfection, Binding Assay, Plasmid Preparation

Figure 4. SIX1 is an insulin-responsive gene and stimulates de novo lipogenesis. A) Lipid droplets (LDs) were visualized using BODIPY 493/503 in SIX1 WT or KO HepG2 cells or SIX1 KO HepG2 cells transfected with SIX1. Scale bar, 20 μm. B) Long-chain fatty acid levels were analyzed by liquid chromatography-mass spectrometry in cells from (A). C) Triglyceride and total cholesterol levels were measured in cells from (A). D-F) Lipid droplets (D), long-chain fatty acid levels (E), and triglyceride and total cholesterol levels (F) were analyzed in WT or HBO1/AIB1/SCD1 KO HepG2 cells transfected with SIX1 or EV. G) Immunoblot analysis of SIX1 WT or KO HepG2 cells treated with insulin (100 nM) or linsitinib (1.0 μM). H, I) Long-chain fatty

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells.

doi: 10.1002/advs.202404229

Figure Lengend Snippet: Figure 4. SIX1 is an insulin-responsive gene and stimulates de novo lipogenesis. A) Lipid droplets (LDs) were visualized using BODIPY 493/503 in SIX1 WT or KO HepG2 cells or SIX1 KO HepG2 cells transfected with SIX1. Scale bar, 20 μm. B) Long-chain fatty acid levels were analyzed by liquid chromatography-mass spectrometry in cells from (A). C) Triglyceride and total cholesterol levels were measured in cells from (A). D-F) Lipid droplets (D), long-chain fatty acid levels (E), and triglyceride and total cholesterol levels (F) were analyzed in WT or HBO1/AIB1/SCD1 KO HepG2 cells transfected with SIX1 or EV. G) Immunoblot analysis of SIX1 WT or KO HepG2 cells treated with insulin (100 nM) or linsitinib (1.0 μM). H, I) Long-chain fatty

Techniques: Transfection, Liquid Chromatography, Mass Spectrometry, Western Blot

Figure 7. Clinical relevance of the DGUOK-AS1/miR-145-5p/SIX1 axis in liver cancer. A) Representative IHC of 73 liver cancer patients. SIX1 and SCD1 were assessed by IHC, and DGUOK-AS1 and miR-145-5p by FISH. Scale bar: 100 μm. The correlations among DGUOK-AS1, miR-145-5p, SCD1, and SIX1 were analyzed as indicated. Case 1 and case 2 refer to 2 representative samples categorized by low and high expression of SIX1. Data was analyzed by Spearman’s Rank Correlation test. B) DGUOK-AS1 expression in 73 cancerous liver tissues and matched adjacent normal liver tissues was determined by FISH. The DGUOK-AS1 expression levels were plotted and compared between normal and cancer tissues (Mann-Whitney U test). C) The disease-free and overall survival curves related to low and high expression of DGUOK-AS1 were analyzed in 73 liver cancer patients from (A) using the Kaplan-Meier method. D) A proposed model underlying the role of the insulin/DGUOK-AS1/miR-145-5p/SIX1 axis in de novo lipogenesis and liver tumor growth and metastasis. Insulin stimulates the expression of DGUOK-AS1, thus sponging miR-145-5p. Inhibition of miR-145-5p promotes expression of SIX1, which recruits histone acetyltransferases AIB1 and HBO1 to induce expression of lipogenic genes (ACLY, FASN, and SCD1). Induction of lipogenic gene expression promotes hepatic lipogenesis and liver tumor growth and metastasis. FAs, fatty acids. TGs, triglycerides. LDs, lipid droplets.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells.

doi: 10.1002/advs.202404229

Figure Lengend Snippet: Figure 7. Clinical relevance of the DGUOK-AS1/miR-145-5p/SIX1 axis in liver cancer. A) Representative IHC of 73 liver cancer patients. SIX1 and SCD1 were assessed by IHC, and DGUOK-AS1 and miR-145-5p by FISH. Scale bar: 100 μm. The correlations among DGUOK-AS1, miR-145-5p, SCD1, and SIX1 were analyzed as indicated. Case 1 and case 2 refer to 2 representative samples categorized by low and high expression of SIX1. Data was analyzed by Spearman’s Rank Correlation test. B) DGUOK-AS1 expression in 73 cancerous liver tissues and matched adjacent normal liver tissues was determined by FISH. The DGUOK-AS1 expression levels were plotted and compared between normal and cancer tissues (Mann-Whitney U test). C) The disease-free and overall survival curves related to low and high expression of DGUOK-AS1 were analyzed in 73 liver cancer patients from (A) using the Kaplan-Meier method. D) A proposed model underlying the role of the insulin/DGUOK-AS1/miR-145-5p/SIX1 axis in de novo lipogenesis and liver tumor growth and metastasis. Insulin stimulates the expression of DGUOK-AS1, thus sponging miR-145-5p. Inhibition of miR-145-5p promotes expression of SIX1, which recruits histone acetyltransferases AIB1 and HBO1 to induce expression of lipogenic genes (ACLY, FASN, and SCD1). Induction of lipogenic gene expression promotes hepatic lipogenesis and liver tumor growth and metastasis. FAs, fatty acids. TGs, triglycerides. LDs, lipid droplets.

Techniques: Expressing, MANN-WHITNEY, Inhibition, Gene Expression

( A ) The experimental paradigm. The differential responses to estradiol (E2) treatment of MCF-7 (cell growth) and long-term estrogen deprived MCF-7:5C cells (apoptosis) are indicated. Proteomics profiles of the two cell lines at baseline and after a brief (2 h) E2 treatment were generated using immunoprecipitations (IP). Proteins interacting with AIB1 or phosphotyrosine containing protein complexes were isolated by IP followed by mass spectrometry. Data were then subjected to an integrated bioinformatics analysis of signaling pathways and protein networks. ( B,C ) Reversal of E2-dependent effects on MCF-7 and MCF-7:5C after depletion of endogenous AIB1 protein using two different lentiviral shRNAs. MCF-7 or MCF-7:5C cells were infected with lentiviral particles expressing control or AIB1-targeting shRNAs. (B) RNAi-mediated knockdown was assayed by Western blot analysis for AIB1 relative to an actin loading control. (C) Cell growth was assayed 6 days after plating without or with E2. The E2 effect is shown relative to the respective untreated controls (mean ±S.E.M.). Closed circles: control shRNA; Open circles (red): AIB1 shRNA. #, p<0.05 E2 treatment effect vs. no treatment in control shRNA cells; *, p<0.05 E2 treatment effect in control shRNA cells vs. E2 treatment in AIB1 depleted cells. Representative data from one of at least three independent experiments are shown.

Journal: PLoS ONE

Article Title: Proteomic Analysis of Pathways Involved in Estrogen-Induced Growth and Apoptosis of Breast Cancer Cells

doi: 10.1371/journal.pone.0020410

Figure Lengend Snippet: ( A ) The experimental paradigm. The differential responses to estradiol (E2) treatment of MCF-7 (cell growth) and long-term estrogen deprived MCF-7:5C cells (apoptosis) are indicated. Proteomics profiles of the two cell lines at baseline and after a brief (2 h) E2 treatment were generated using immunoprecipitations (IP). Proteins interacting with AIB1 or phosphotyrosine containing protein complexes were isolated by IP followed by mass spectrometry. Data were then subjected to an integrated bioinformatics analysis of signaling pathways and protein networks. ( B,C ) Reversal of E2-dependent effects on MCF-7 and MCF-7:5C after depletion of endogenous AIB1 protein using two different lentiviral shRNAs. MCF-7 or MCF-7:5C cells were infected with lentiviral particles expressing control or AIB1-targeting shRNAs. (B) RNAi-mediated knockdown was assayed by Western blot analysis for AIB1 relative to an actin loading control. (C) Cell growth was assayed 6 days after plating without or with E2. The E2 effect is shown relative to the respective untreated controls (mean ±S.E.M.). Closed circles: control shRNA; Open circles (red): AIB1 shRNA. #, p<0.05 E2 treatment effect vs. no treatment in control shRNA cells; *, p<0.05 E2 treatment effect in control shRNA cells vs. E2 treatment in AIB1 depleted cells. Representative data from one of at least three independent experiments are shown.

Article Snippet: Western blot analyses were done as previously described , using a monoclonal antibody for AIB1 (SRC3; clone 5E11, Cell Signaling).

Techniques: Generated, Isolation, Mass Spectrometry, Infection, Expressing, Western Blot, shRNA

Venn diagrams of proteins identified from anti-AIB1 ( A,C ) or anti-pY IP ( B,D ) experimental groups. ( C,D ) Proteins in combined AIB1-IP or pY-IP data sets. Individual proteins and subgroups are shown in & .

Journal: PLoS ONE

Article Title: Proteomic Analysis of Pathways Involved in Estrogen-Induced Growth and Apoptosis of Breast Cancer Cells

doi: 10.1371/journal.pone.0020410

Figure Lengend Snippet: Venn diagrams of proteins identified from anti-AIB1 ( A,C ) or anti-pY IP ( B,D ) experimental groups. ( C,D ) Proteins in combined AIB1-IP or pY-IP data sets. Individual proteins and subgroups are shown in & .

Article Snippet: Western blot analyses were done as previously described , using a monoclonal antibody for AIB1 (SRC3; clone 5E11, Cell Signaling).

Techniques:

The thick grey line in the middle provides an arbitrary boundary between the pathways. Anti-AIB1 immunoprecipitated (AIB1-IPed) and anti-pY-immunoprecipitated proteins (pY-IPed) are indicated by red or green circles respectively (keys at the bottom). The blue circled proteins are AIB1-IPed proteins from MCF-7 (CALM1) or MCF-7:5C cells (β-catenin) under both E2-treated and untreated conditions; the purple circled one (ITPR3) is an AIB1-IPed protein from both cells only under E2 treated condition, while the yellow circled one (TYK2) is an AIB1-IPed protein from both cells under both E2 treated and untreated conditions. Proteins circled in grey are from known canonical pathways (e.g. ERK in cell growth or BAD in apoptosis) but not identified here. Solid line arrows indicate direct interactions (e.g. CDK1 phosphorylates Rap1GAP) or translocations (e.g. catenins) of proteins, while dashed arrows indicate indirect actions of proteins (e.g. AKT activate MEK through several steps). Hammer-ended lines indicate inhibitory effects on the target. Detailled pathways are shown in , , , .

Journal: PLoS ONE

Article Title: Proteomic Analysis of Pathways Involved in Estrogen-Induced Growth and Apoptosis of Breast Cancer Cells

doi: 10.1371/journal.pone.0020410

Figure Lengend Snippet: The thick grey line in the middle provides an arbitrary boundary between the pathways. Anti-AIB1 immunoprecipitated (AIB1-IPed) and anti-pY-immunoprecipitated proteins (pY-IPed) are indicated by red or green circles respectively (keys at the bottom). The blue circled proteins are AIB1-IPed proteins from MCF-7 (CALM1) or MCF-7:5C cells (β-catenin) under both E2-treated and untreated conditions; the purple circled one (ITPR3) is an AIB1-IPed protein from both cells only under E2 treated condition, while the yellow circled one (TYK2) is an AIB1-IPed protein from both cells under both E2 treated and untreated conditions. Proteins circled in grey are from known canonical pathways (e.g. ERK in cell growth or BAD in apoptosis) but not identified here. Solid line arrows indicate direct interactions (e.g. CDK1 phosphorylates Rap1GAP) or translocations (e.g. catenins) of proteins, while dashed arrows indicate indirect actions of proteins (e.g. AKT activate MEK through several steps). Hammer-ended lines indicate inhibitory effects on the target. Detailled pathways are shown in , , , .

Article Snippet: Western blot analyses were done as previously described , using a monoclonal antibody for AIB1 (SRC3; clone 5E11, Cell Signaling).

Techniques: Immunoprecipitation

Journal: PLoS ONE

Article Title: Proteomic Analysis of Pathways Involved in Estrogen-Induced Growth and Apoptosis of Breast Cancer Cells

doi: 10.1371/journal.pone.0020410

Article Snippet: For the mass spectrometry analysis, protein lysates from cells treated for 2 hours with E2 or vehicle were subjected to immunoprecipitation using gamma-bind G-Sepharose beads and an anti-AIB1 monoclonal antibody (BD Biosciences) as described or an anti-phosphotyrosine monoclonal antibody (4G-10, Millipore).

Techniques: Generated, Isolation, Mass Spectrometry, Infection, Expressing, Western Blot, shRNA

Journal: PLoS ONE

Article Title: Proteomic Analysis of Pathways Involved in Estrogen-Induced Growth and Apoptosis of Breast Cancer Cells

doi: 10.1371/journal.pone.0020410

Techniques:

Journal: PLoS ONE

Article Title: Proteomic Analysis of Pathways Involved in Estrogen-Induced Growth and Apoptosis of Breast Cancer Cells

doi: 10.1371/journal.pone.0020410

Techniques: Immunoprecipitation

Clinicopathological features of patients with UTUC

Journal: Cancer Management and Research

Article Title: Prognostic value of AIB1 and EIF5A2 in intravesical recurrence after surgery for upper tract urothelial carcinoma

doi: 10.2147/CMAR.S185392

Figure Lengend Snippet: Clinicopathological features of patients with UTUC

Article Snippet: The slides were incubated with the anti-AIB1 antibody (BD Transduction Laboratories; Lexington, KY, USA; diluted 1:100 in PBS) or anti-EIF5A2 antibody (Abnova, Jhongli, Taiwan; diluted 1:100 in PBS) in a moist chamber.

Techniques: Expressing

Immunohistochemical expression of AIB1 and EIF5A2 in upper tract urothelial carcinoma tissue (×200). Notes: ( A ) AIB1 score 0 points (negative staining), ( B ) low expression of AIB1 (score 3 points), ( C ) high expression of AIB1 (score 9 points), ( D ) EIF5A2 score 0 points (negative staining), ( E ) low expression of EIF5A2 (score 4 points) and ( F ) high expression of EIF5A2 (score 12 points).

Journal: Cancer Management and Research

Article Title: Prognostic value of AIB1 and EIF5A2 in intravesical recurrence after surgery for upper tract urothelial carcinoma

doi: 10.2147/CMAR.S185392

Figure Lengend Snippet: Immunohistochemical expression of AIB1 and EIF5A2 in upper tract urothelial carcinoma tissue (×200). Notes: ( A ) AIB1 score 0 points (negative staining), ( B ) low expression of AIB1 (score 3 points), ( C ) high expression of AIB1 (score 9 points), ( D ) EIF5A2 score 0 points (negative staining), ( E ) low expression of EIF5A2 (score 4 points) and ( F ) high expression of EIF5A2 (score 12 points).

Techniques: Immunohistochemical staining, Expressing, Negative Staining

Association of AIB1 and EIF5A2 expression with clinicopathological parameters in 109 patients with UTUC

Journal: Cancer Management and Research

Article Title: Prognostic value of AIB1 and EIF5A2 in intravesical recurrence after surgery for upper tract urothelial carcinoma

doi: 10.2147/CMAR.S185392

Figure Lengend Snippet: Association of AIB1 and EIF5A2 expression with clinicopathological parameters in 109 patients with UTUC

Techniques: Expressing

Kaplan–Meier plots show intravesical recurrence-free survival curves according to AIB1 expression status ( A ), low AIB1 expression (blue line), n=58; high AIB1 expression (green line), n=51. EIF5A2 expression status ( B ), low EIF5A2 expression (blue line), n=61; high EIF5A2 expression (green line), n=48.

Journal: Cancer Management and Research

Article Title: Prognostic value of AIB1 and EIF5A2 in intravesical recurrence after surgery for upper tract urothelial carcinoma

doi: 10.2147/CMAR.S185392

Figure Lengend Snippet: Kaplan–Meier plots show intravesical recurrence-free survival curves according to AIB1 expression status ( A ), low AIB1 expression (blue line), n=58; high AIB1 expression (green line), n=51. EIF5A2 expression status ( B ), low EIF5A2 expression (blue line), n=61; high EIF5A2 expression (green line), n=48.

Techniques: Expressing

Kaplan–Meier survival analysis of AIB1 expression in subsets of different grades, pT and pN patients with UTUC (log-rank test). Notes: ( A ) Low-grade, probability of IVRFS of low-grade patients with UTUC: low expression (blue line), n=20; high expression (green line), n=18. ( B ) High-grade, probability of IVRFS of high-grade patients with UTUC: low expression (blue line), n=38; high expression (green line), n=33. ( C ) pTa-1, probability of IVRFS of pTa-1 patients with UTUC: low expression (blue line), n=21; high expression (green line), n=16. ( D ) pT2–4, probability of IVRFS of pT2–4 patients with UTUC: low expression (blue line), n=37; high expression (green line), n=35. ( E ) pN0, probability of IVRFS of pN0 patients with UTUC: low expression (blue line), n=53; high expression (green line), n=40. ( F ) pN1–2, probability of IVRFS of pN1–2 patients with UTUC: low expression (blue line), n=5; high expression (green line), n=11. Abbreviations: IVRFS, intravesical recurrence-free survival; UTUC, upper tract urothelial carcinoma.

Journal: Cancer Management and Research

Article Title: Prognostic value of AIB1 and EIF5A2 in intravesical recurrence after surgery for upper tract urothelial carcinoma

doi: 10.2147/CMAR.S185392

Figure Lengend Snippet: Kaplan–Meier survival analysis of AIB1 expression in subsets of different grades, pT and pN patients with UTUC (log-rank test). Notes: ( A ) Low-grade, probability of IVRFS of low-grade patients with UTUC: low expression (blue line), n=20; high expression (green line), n=18. ( B ) High-grade, probability of IVRFS of high-grade patients with UTUC: low expression (blue line), n=38; high expression (green line), n=33. ( C ) pTa-1, probability of IVRFS of pTa-1 patients with UTUC: low expression (blue line), n=21; high expression (green line), n=16. ( D ) pT2–4, probability of IVRFS of pT2–4 patients with UTUC: low expression (blue line), n=37; high expression (green line), n=35. ( E ) pN0, probability of IVRFS of pN0 patients with UTUC: low expression (blue line), n=53; high expression (green line), n=40. ( F ) pN1–2, probability of IVRFS of pN1–2 patients with UTUC: low expression (blue line), n=5; high expression (green line), n=11. Abbreviations: IVRFS, intravesical recurrence-free survival; UTUC, upper tract urothelial carcinoma.

Techniques: Expressing

Kaplan–Meier survival analysis of postoperative intravesical chemotherapy in subsets of different expression of AIB1 and EIF5A2 (log-rank test). Notes: AIB1 (−): low AIB1 expression, AIB1(+): high AIB1 expression, EIF5A2 (−): low EIF5A2 expression, and EIF5A2 (+): high EIF5A2 expression. ( A ) AIB1(−) EIF5A2(−), probability of IVRFS of intravesical chemotherapy for patients with UTUC: no instillation (blue line), n=1; instillation (green line), n=29. ( B ) AIB1 (−) EIF5A2 (+), probability of IVRFS of intravesical chemotherapy for patients with UTUC: no instillation (blue line), n=5; instillation (green line), n=23. ( C ) AIB1(+)EIF5A2(−), probability of IVRFS of intravesical chemotherapy for patients with UTUC: no instillation (blue line), n=10; instillation (green line), n=21. ( D ) AIB1(+)EIF5A2(+), probability of IVRFS of intravesical chemotherapy for patients with UTUC: no instillation (blue line), n=6; instillation (green line), n=14. Abbreviations: IVRFS, intravesical recurrence-free survival; UTUC, upper tract urothelial carcinoma.

Journal: Cancer Management and Research

Article Title: Prognostic value of AIB1 and EIF5A2 in intravesical recurrence after surgery for upper tract urothelial carcinoma

doi: 10.2147/CMAR.S185392

Figure Lengend Snippet: Kaplan–Meier survival analysis of postoperative intravesical chemotherapy in subsets of different expression of AIB1 and EIF5A2 (log-rank test). Notes: AIB1 (−): low AIB1 expression, AIB1(+): high AIB1 expression, EIF5A2 (−): low EIF5A2 expression, and EIF5A2 (+): high EIF5A2 expression. ( A ) AIB1(−) EIF5A2(−), probability of IVRFS of intravesical chemotherapy for patients with UTUC: no instillation (blue line), n=1; instillation (green line), n=29. ( B ) AIB1 (−) EIF5A2 (+), probability of IVRFS of intravesical chemotherapy for patients with UTUC: no instillation (blue line), n=5; instillation (green line), n=23. ( C ) AIB1(+)EIF5A2(−), probability of IVRFS of intravesical chemotherapy for patients with UTUC: no instillation (blue line), n=10; instillation (green line), n=21. ( D ) AIB1(+)EIF5A2(+), probability of IVRFS of intravesical chemotherapy for patients with UTUC: no instillation (blue line), n=6; instillation (green line), n=14. Abbreviations: IVRFS, intravesical recurrence-free survival; UTUC, upper tract urothelial carcinoma.

Techniques: Expressing

Univariate and multivariate Cox regression models predicting bladder recurrence

Journal: Cancer Management and Research

Article Title: Prognostic value of AIB1 and EIF5A2 in intravesical recurrence after surgery for upper tract urothelial carcinoma

doi: 10.2147/CMAR.S185392

Figure Lengend Snippet: Univariate and multivariate Cox regression models predicting bladder recurrence

Techniques: Expressing

Kaplan–Meier survival analysis of postoperative intravesical chemotherapy in subsets of different risk groups of the prognostic model including EIF5A2, AIB1, lymph node status and RNU (log-rank test). Note: Probability of IVRFS of all cases: no instillation (blue line), n=22; instillation (green line), n=87 ( A ); low-risk, probability of IVRFS of the low-risk group: no instillation (blue line), n=9; instillation (green line), n=46 ( B ); high-risk, probability of IVRFS of high-risk group: no instillation (blue line), n=13; instillation (green line), n=41 ( C ). Abbreviations: IVRFS, intravesical recurrence-free survival; R N U, radical nephroureterectomy.

Journal: Cancer Management and Research

Article Title: Prognostic value of AIB1 and EIF5A2 in intravesical recurrence after surgery for upper tract urothelial carcinoma

doi: 10.2147/CMAR.S185392

Figure Lengend Snippet: Kaplan–Meier survival analysis of postoperative intravesical chemotherapy in subsets of different risk groups of the prognostic model including EIF5A2, AIB1, lymph node status and RNU (log-rank test). Note: Probability of IVRFS of all cases: no instillation (blue line), n=22; instillation (green line), n=87 ( A ); low-risk, probability of IVRFS of the low-risk group: no instillation (blue line), n=9; instillation (green line), n=46 ( B ); high-risk, probability of IVRFS of high-risk group: no instillation (blue line), n=13; instillation (green line), n=41 ( C ). Abbreviations: IVRFS, intravesical recurrence-free survival; R N U, radical nephroureterectomy.

Techniques:

ROC curves comparing the predictive accuracy by the combined AIB1, EIF5A2, pN, and the surgical approach of RNU, the AIB1 alone model, the EIF5A2 alone model, the pN model, and RNU alone model for intravesical recurrence-free survival. Abbreviations: RNU, radical nephroureterectomy; ROC, receiver operating characteristic.

Journal: Cancer Management and Research

Article Title: Prognostic value of AIB1 and EIF5A2 in intravesical recurrence after surgery for upper tract urothelial carcinoma

doi: 10.2147/CMAR.S185392

Figure Lengend Snippet: ROC curves comparing the predictive accuracy by the combined AIB1, EIF5A2, pN, and the surgical approach of RNU, the AIB1 alone model, the EIF5A2 alone model, the pN model, and RNU alone model for intravesical recurrence-free survival. Abbreviations: RNU, radical nephroureterectomy; ROC, receiver operating characteristic.

Techniques:

Clinicopathologic correlation of AIB1 expression in cervical cancer.

Journal: Journal of Cancer

Article Title: AIB1 predicts tumor response to definitive chemoradiotherapy and prognosis in cervical squamous cell carcinoma

doi: 10.7150/jca.31697

Figure Lengend Snippet: Clinicopathologic correlation of AIB1 expression in cervical cancer.

Article Snippet: Equal amounts of cell lysates were resolved by SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Millipore, Bed-ford, MA, USA) followed by incubating with primary antibodies against AIB1 (BD Transduction Laboratories); cleaved-caspase 3 (Asp175), (Cell Signaling Technology, Boston.

Techniques: Expressing

Kaplan-Meier survival analysis of the AIB1 expression in total cohort and different subsets of cervical cancer patients. A , Total cohort (high expression =57cases, low expression=51 cases). B , M0 subset (high =38 cases, low = 43 cases). C , M1 subset (high =19 cases, low = 8 cases). D , N0 subset (high =32 cases, low = 39 cases). E , non-CR subset (high =39 cases, low = 23 cases). F , III+IV subset (high = 49 cases, low = 27 cases). The P value was calculated using a log-rank test.

Journal: Journal of Cancer

Article Title: AIB1 predicts tumor response to definitive chemoradiotherapy and prognosis in cervical squamous cell carcinoma

doi: 10.7150/jca.31697

Figure Lengend Snippet: Kaplan-Meier survival analysis of the AIB1 expression in total cohort and different subsets of cervical cancer patients. A , Total cohort (high expression =57cases, low expression=51 cases). B , M0 subset (high =38 cases, low = 43 cases). C , M1 subset (high =19 cases, low = 8 cases). D , N0 subset (high =32 cases, low = 39 cases). E , non-CR subset (high =39 cases, low = 23 cases). F , III+IV subset (high = 49 cases, low = 27 cases). The P value was calculated using a log-rank test.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: AIB1 predicts tumor response to definitive chemoradiotherapy and prognosis in cervical squamous cell carcinoma

doi: 10.7150/jca.31697

Figure Lengend Snippet: Multivariate Cox regression analysis.

Techniques: Expressing

Expression of AIB1 in cervical cancer. A , The levels of AIB1 proteins in three cervical cancer cell lines and non-neoplastic cervical benign tissues examined by western blot. B , Normal cervical epithelial tissues (case 30) showed normal expression of AIB1 protein with a negative staining of AIB1 in the nuclei of all cervical epithelial cells (200×). C , Cervical squamous cell carcinoma (case 17) demonstrated normal expression of AIB1, in which all tumor cells showed negative staining of AIB1 (200×). D , Low expression of AIB1 was detected in cervical squamous cell carcinoma (case 34), in which less than 10% cancer cells showed low staining of AIB1 protein in the nuclei (200×). E , High expression of AIB1 was observed in cervical squamous cell carcinoma (case 21), in which 10~70% cancer cells demonstrated positive staining of AIB1 in the nuclei (200×). F , Another cervical squamous cell carcinoma (case 36) showed high expression of AIB1, in which more than 70% cancer cells showed high staining of AIB1 protein in the nuclei (200×).

Journal: Journal of Cancer

Article Title: AIB1 predicts tumor response to definitive chemoradiotherapy and prognosis in cervical squamous cell carcinoma

doi: 10.7150/jca.31697

Figure Lengend Snippet: Expression of AIB1 in cervical cancer. A , The levels of AIB1 proteins in three cervical cancer cell lines and non-neoplastic cervical benign tissues examined by western blot. B , Normal cervical epithelial tissues (case 30) showed normal expression of AIB1 protein with a negative staining of AIB1 in the nuclei of all cervical epithelial cells (200×). C , Cervical squamous cell carcinoma (case 17) demonstrated normal expression of AIB1, in which all tumor cells showed negative staining of AIB1 (200×). D , Low expression of AIB1 was detected in cervical squamous cell carcinoma (case 34), in which less than 10% cancer cells showed low staining of AIB1 protein in the nuclei (200×). E , High expression of AIB1 was observed in cervical squamous cell carcinoma (case 21), in which 10~70% cancer cells demonstrated positive staining of AIB1 in the nuclei (200×). F , Another cervical squamous cell carcinoma (case 36) showed high expression of AIB1, in which more than 70% cancer cells showed high staining of AIB1 protein in the nuclei (200×).

Techniques: Expressing, Western Blot, Negative Staining, Staining

Lentivirus-mediated AIB1 silencing enhances the chemosensitivities of cervical cancer cells. A - D , Dose-response curves of cisplatin or 5-Fu in SiHa and CaSki cells. AIB1-shRNA infected SiHa and CaSki cells showed more sensitive to cisplatin and 5-Fu than parental control cells. IC50 values are shown below. Data are Mean ± SD (n=3, P<0.05). E and F , After treated cells with cisplatin (IC30) or 5-Fu (IC30) for the 24 hours, the cleaved PARP and cleaved caspase-3 were detected in AIB1-shRNA and Luc-shRNA infected SiHa and CaSki cells by western blot.

Journal: Journal of Cancer

Article Title: AIB1 predicts tumor response to definitive chemoradiotherapy and prognosis in cervical squamous cell carcinoma

doi: 10.7150/jca.31697

Figure Lengend Snippet: Lentivirus-mediated AIB1 silencing enhances the chemosensitivities of cervical cancer cells. A - D , Dose-response curves of cisplatin or 5-Fu in SiHa and CaSki cells. AIB1-shRNA infected SiHa and CaSki cells showed more sensitive to cisplatin and 5-Fu than parental control cells. IC50 values are shown below. Data are Mean ± SD (n=3, P<0.05). E and F , After treated cells with cisplatin (IC30) or 5-Fu (IC30) for the 24 hours, the cleaved PARP and cleaved caspase-3 were detected in AIB1-shRNA and Luc-shRNA infected SiHa and CaSki cells by western blot.

Techniques: shRNA, Infection, Western Blot

Comparison of radiobiological parameters of SiHa cells infected with sh-luc or sh-AIB1.

Journal: Journal of Cancer

Article Title: AIB1 predicts tumor response to definitive chemoradiotherapy and prognosis in cervical squamous cell carcinoma

doi: 10.7150/jca.31697

Figure Lengend Snippet: Comparison of radiobiological parameters of SiHa cells infected with sh-luc or sh-AIB1.

Techniques: Infection

AIB1 depletion increases the sensitivity of cervical cancer cells in response to ionizing radiation. A , Clonogenic survival of SiHa-shAIB1 and SiHa-shluc cells. Cells were exposed to increasing doses of radiation as indicated. After 12 days, colonies more than 50 cells were counted and survival curves were fitted according to the linear-quadratic mode. Results were obtained from three independent experiments. All results were from three independent experiments. B , After irradiated shAIB1-Siha and shluc-Siha cells with 3Gy or 0 Gy x-ray, the cleaved caspase-3 and cleaved PARP were detected by Western blot analysis. Experiments were performed three times and a representative result is shown. C , SiHa-shAIB1 and SiHa-shluc cells were initally treated with or without IR. 36 hours later, the proportion of apoptotic cells were determined by PI/Annexin V assay. Data described are the Means ± SD of triplicates (* P <0.05, Student's t-test).

Journal: Journal of Cancer

Article Title: AIB1 predicts tumor response to definitive chemoradiotherapy and prognosis in cervical squamous cell carcinoma

doi: 10.7150/jca.31697

Figure Lengend Snippet: AIB1 depletion increases the sensitivity of cervical cancer cells in response to ionizing radiation. A , Clonogenic survival of SiHa-shAIB1 and SiHa-shluc cells. Cells were exposed to increasing doses of radiation as indicated. After 12 days, colonies more than 50 cells were counted and survival curves were fitted according to the linear-quadratic mode. Results were obtained from three independent experiments. All results were from three independent experiments. B , After irradiated shAIB1-Siha and shluc-Siha cells with 3Gy or 0 Gy x-ray, the cleaved caspase-3 and cleaved PARP were detected by Western blot analysis. Experiments were performed three times and a representative result is shown. C , SiHa-shAIB1 and SiHa-shluc cells were initally treated with or without IR. 36 hours later, the proportion of apoptotic cells were determined by PI/Annexin V assay. Data described are the Means ± SD of triplicates (* P <0.05, Student's t-test).

Techniques: Irradiation, Western Blot, Annexin V Assay

Review

aib1 antibody (Cell Signaling Technology Inc)

Structured Review

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1) Product Images from "An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progression"

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